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What factors affect the resolution and retention time in HPLC?
In High-Performance Liquid Chromatography (HPLC), resolution (how well two peaks are separated) and retention time (how long a compound takes [...]
What TLC plates are best for separating highly polar compounds?
For separating highly polar compounds on TLC, reversed-phase plates are typically the most effective choice. These plates feature a [...]
Protein Desalting Columns: A Small Step with a Big Impact in Your Chromatographic Toolbox
Protein Desalting Columns: A Small Step with a Big Impact in Your Chromatographic Toolbox In protein purification workflows, it’s [...]
Flash Cartridge Compatibility with Automated Chromatography Systems
When it comes to flash chromatography, one of the most common frustrations researchers face is cartridge compatibility. Many labs [...]
Macherey‑Nagel C18 SPE Cartridges: High‑Purity Silica Solutions for Reliable and Efficient Sample Preparation
Macherey‑Nagel C18 SPE Cartridges: High‑Purity Silica Solutions for Reliable and Efficient Sample Preparation Why Macherey‑Nagel C18 SPE Cartridges Deliver Superior [...]
Mastering Stationary Phases: Selection Criteria and Method Development
Selection Criteria and Method Development By Ray Lombardi, Marc Perla, Rob Cotta, and Robert R. Kerr Ph.D. [...]
Flash Chromatography Basics
Instructions for Sample Preparation Start with TLC Screening Use Thin Layer Chromatography (TLC) to identify a solvent or solvent [...]
How to Identify Peaks in a Chromatogram and Assign Them to the Correct Analyte
How to Identify Peaks in a Chromatogram Chromatography is one of the core tools in analytical chemistry. It allows [...]