Thin Layer Chromatography, TLC Plates



Normal Phase Reverse Phase Analytical TLC Plates Prep TLC Plates
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A Premier TLC Plate Vendor
As a premier TLC Plate vendor, Sorbent Technologies offers the highest performance and widest selection of TLC plates on glass, plastic, and aluminum supports. Analytical and preparative layers with different sorbent coatings: silica gel, alumina, cellulose, bonded phases, polyamide, chiral, and more are available. TLC plates are available in a wide array of sizes from 20 x 20cm to 2.5 x 7.5cm with or without UV254 indicator and/or gypsum binder (11%).

Our TLC plates are guaranteed by stringent production control including standardized lot tests, surface checks for roughness or cracks as well as hardness and adherence checks. Plates and sheets are immediately ready for chromatographic separation and require no coatings or impregnations.TLC layers are smooth, homogenous and well-adhering to produce reproducible quantitative evaluations.

Thin layer chromatography, TLC Plates

Sizes Available: Many pre-cut sizes are available as shown in photo.

  • Analytical TLC Layer thickness: 50µm, 100µm, 150µm, 200µm, 250µm
  • Preparative TLC Layer thickness: 500µm, 1000µm, and 2000µm
  • Available with or without UV254 indicator.

Available TLC Backings
There are three supports used in TLC plates: glass, plastic, and aluminum. There are advantages and disadvantages to each, so it is important to consider these differences when determining what backing is appropriate for your TLC needs.

  1. Glass-Backed TLC Plates: The support used most often in TLC is the glass plate due to its chemical resistance and the ease of handling the plate. The disadvantages of glass plates are its fragility, size and weight (glass plates are about 1.3 mm thick) and require additional protective packaging material.

  2. Plastic-Backed TLC Plates: Polyester sheets (about 0.2 mm thick) have several advantages: They are resistant towards all common solvents, need less packing and require less shelf space for storage. The plastic backed TLC plates can be easily cut with scissors into any required size, and the spots can be cut and eluted. Charring techniques can be applied for silica coated plastic TLC plates. The charring has to be done at lower temperatures than on glass. The maximum temperature for plastic TLC plates is 160°C.

  3. Aluminum-Backed TLC Plates: Because of a thicker aluminum support (about 0.15 mm thick), aluminum-backed TLC plates are much more stable and easier to cut than glass or plastic backed TLC plates. Even a 20 x 20 cm Aluminum backed TLC plate has a torsional strength that is almost as good as a glass plate. The aluminum-backed TLC layer features an outstanding adherence, which allows cutting of the sheets to any size desired without disturbing the sorbent layer. A support of plain aluminum foil is coated using the same time-tested binder system as for our TLC glass plates. For eluents containing high concentrations of water, we recommend our pre-coated Aluminum TLC plates.

Like plastic backed TLC plates sheets, glass and aluminum TLC plates can be sprayed or dipped with purely aqueous visualization reagents without any significant damage to the layer. With aluminum backed TLC plates, problems might arise when using eluents containing high concentrations of mineral acids or concentrated ammonia (reacts with aluminum). As a solution to the issue of backing instability, we recommend using our glass or polyester TLC plates. Aluminum backed TLC plates offer the same advantages as the plastic backed TLC plates with increased temperature resistance (e.g. for charring techniques).