Chromabond® QuEChERS
Optimized Solutions for Pesticide Residue Analysis
QuEChERS methodology is defined as “Quick, Easy, Cheap, Effective, Rugged and Safe“ – these are the demands of sample preparation in modern food analysis. QuEChERS have become the method of choice in sample preparation for the analysis of pesticides and drugs in fruit, vegetables, and other food products.
The QuEChERS method is used to determine pesticide residues in food samples by GC-MS or LC-MS, allowing:
- Rapid clean-up of strongly matrix-contaminated samples
- Extraction optimization of pH-dependent compounds
- Minimizing the decomposition of sensitive substances
- Broadening the matrix spectrum
Advantages of QuEChERS in comparison
with classical clean-up methods:
with classical clean-up methods:
- Easy to handle and time-saving procedure
- No need for glassware
- Low consumption of solvents
- No need for chlorinated solvents
- Broad range of pesticides can be detected
- Rugged method with high and safe recovery rates
In addition to the required adsorbent, CHROMABOND® Diamino offers several individually weighed and premixed buffer and extraction mixtures, specially composed for different sample matrices.
The European standard EN 15662 recommends a citrate extraction mix (Mix I), while AOAC standard 2007.1 uses an acetate extraction mix (Mix II).
For clean-up, the Diamino phase (PSA) removes, e.g., sugars and organic acids. Magnesium sulfate removes water, C18ec removes nonpolar interferences, such as fats and the carbon phase removes pigments, sterols, and nonpolar interferences.
Chromabond® QuEChERS Properties:
- Base material silica, pore size 60 Å, particle size 45 µm, specific surface 500 m2/g, pH stability 2–8
- Primary and secondary amine functions (PSA), 5 % C
- Removes polar compounds (e.g., organic acids, pigments, sugars) from matrices like fruit or vegetables
- Similar phases to Supelcleantm PSA, Bond Eluttm PSA
| Adsorbents and What They Are Used For | |
|---|---|
| MgSO4 | Removes excess of water |
| NaCl | For phase separation |
| CHROMABOND® Diamino (PSA) (Primary Secondary Amine) | Removes organic and fatty acids, sugars and anthocyanin pigments |
| CHROMABOND® C18 ec (Reversed phase modified silica) | Traps nonpolar compounds, e.g., lipids |
| CHROMABOND® Carbon (GCB) (Graphitized Carbon Black) |
Removes pigments and sterols. Note: planar pesticides are also removed |
| Catalog No. | Description | Price (USD) |
|---|---|---|
| 730970 | Chromabond QuEChERS, Mix I Citrate extraction mix 50/pk |  Buy now $160.00 |
| 730971 | Chromabond QuEChERS, Mix II Acetate extraction mix 50/pk | Buy now $160.00 |
| 730972 | Chromabond QuEChERS, Mix III Diamino clean-up mix 50/pk | Buy now $108.00 |
| 730973 | Chromabond QuEChERS, Mix IV Diamino/Carbon clean-up mix 50/pk | Buy now $134.00 |
| 730975 | Chromabond QuEChERS, Mix V Diamino/Carbon clean-up mix 50/pk | Buy now $134.00 |
| 730974 | Chromabond QuEChERS, Mix VI Diamino/C18 ec clean-up mix 50/pk | Buy now $108.00 |
| QuEChERS Mixes | ||
|---|---|---|
| Sample Property | QuEChERS Extraction Mixes | QuEChERS clean-up mixes |
| Low fat content (e.g., Apples, Strawberries) |
Citrate or acetate extraction Mix I or Mix II |
Diamino clean-up Mix III |
| Moderate content of chlorophyll and carotinoids (e.g., Carrots, Lettuce) |
Citrate or acetate extraction Mix I or Mix II |
Diamino/Carbon clean-up Mix IV |
| Higher content of chlorophyll and carotinoids (e.g., Bell Peppers, Spinach) |
Citrate extraction Mix I |
Diamino/Carbon clean-up (higher Carbon content) Mix V |
| Higher fat content (e.g., Avocado) |
Citrate extraction Mix I |
Diamino/C18 ec clean-up |
| CHROMABOND® QuEChERS Extraction Buffer Mixes | ||||
|---|---|---|---|---|
| Catalog No. | Description | Composition | Volume | Qty/pkg |
| 730970 | Mix I Citrate Extraction Mix | 4g MgSO₄, 1g NaCl, 0.5g Na₂H Citrate 1.5 H₂O, 1g Na₃ Citrate 2 H₂O | 15 ml | 50 |
| 730971 | Mix II Acetate Extraction Mix | 6g MgSO₄, 1.5g Na Citrate | 15 ml | 50 |
| CHROMABOND® QuEChERS Clean Up Mixes | ||||
|---|---|---|---|---|
| Catalog No. | Description | Composition | Volume | Qty/pkg |
| 730972 | Mix III Diamino Clean Up Mix | 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ | 15 ml | 50 |
| 730973 | Mix IV Diamino/Carbon Clean Up Mix | 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ and 15 mg Carbon | 15 ml | 50 |
| 730974 | Mix IV Diamino/Carbon Clean Up Mix | 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ and 45 mg Carbon | 15 ml | 50 |
| 730975 | Mix VI Diamino/C18 ec Clean Up Mix | 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ and 150 mg C18 ec | 15 ml | 50 |
| CHROMABOND® Diamino Polypropylene SPE Cartridges | ||||
|---|---|---|---|---|
| Catalog No. | Description | Weight | Size | Qty/pkg |
| 730561 | Diamino Adsorbent | 200 mg | 3 ml | 50 |
| 730562 | Diamino Adsorbent | 500 mg | 6 ml | 30 |
| CHROMABOND® Diamino Adsorbents | ||
|---|---|---|
| Catalog No. | Description | Qty/pkg |
| 730653.20 | Diamino Adsorbent | 20g |
| 730653 | Diamino Adsorbent | 100g |