Chromabond® QuEChERS

Optimized Solutions for Pesticide Residue Analysis

QuEChERS methodology is defined as “Quick, Easy, Cheap, Effective, Rugged and Safe“ – these are the demands of sample preparation in modern food analysis. QuEChERS have become the method of choice in sample preparation for the analysis of pesticides and drugs in fruit, vegetables, and other food products.

Chromabond QuEChERS

The QuEChERS method is used to determine pesticide residues in food samples by GC-MS or LC-MS, allowing:

  • Rapid clean-up of strongly matrix-contaminated samples
  • Extraction optimization of pH-dependent compounds
  • Minimizing the decomposition of sensitive substances
  • Broadening the matrix spectrum

Advantages of QuEChERS in comparison
with classical clean-up methods:

  • Easy to handle and time-saving procedure
  • No need for glassware
  • Low consumption of solvents
  • No need for chlorinated solvents
  • Broad range of pesticides can be detected
  • Rugged method with high and safe recovery rates

In addition to the required adsorbent, CHROMABOND® Diamino offers several individually weighed and premixed buffer and extraction mixtures, specially composed for different sample matrices.

The European standard EN 15662 recommends a citrate extraction mix (Mix I), while AOAC standard 2007.1 uses an acetate extraction mix (Mix II).

For clean-up, the Diamino phase (PSA) removes, e.g., sugars and organic acids. Magnesium sulfate removes water, C18ec removes nonpolar interferences, such as fats and the carbon phase removes pigments, sterols, and nonpolar interferences.

Chromabond® QuEChERS Properties:

  • Base material silica, pore size 60 Å, particle size 45 µm, specific surface 500 m2/g, pH stability 2–8
  • Primary and secondary amine functions (PSA), 5 % C
  • Removes polar compounds (e.g., organic acids, pigments, sugars) from matrices like fruit or vegetables
  • Similar phases to Supelcleantm PSA, Bond Eluttm PSA
quechers tube
Adsorbents and What They Are Used For
MgSO4 Removes excess of water
NaCl For phase separation
CHROMABOND® Diamino (PSA) (Primary Secondary Amine) Removes organic and fatty acids, sugars and anthocyanin pigments
CHROMABOND® C18 ec (Reversed phase modified silica) Traps nonpolar compounds, e.g., lipids
CHROMABOND® Carbon (GCB) (Graphitized Carbon Black) Removes pigments and sterols.
Note: planar pesticides are also removed
Chromabond QuEChERS 2
Catalog No.DescriptionPrice (USD)
730970Chromabond QuEChERS, Mix I Citrate extraction mix 50/pk Buy now $147.00
730971Chromabond QuEChERS, Mix II Acetate extraction mix 50/pk Buy now $147.00
730972Chromabond QuEChERS, Mix III Diamino clean-up mix 50/pk Buy now $99.00
730973Chromabond QuEChERS, Mix IV Diamino/Carbon clean-up mix 50/pk Buy now $123.00
730975Chromabond QuEChERS, Mix V Diamino/Carbon clean-up mix 50/pk Buy now $123.00
730974Chromabond QuEChERS, Mix VI Diamino/C18 ec clean-up mix 50/pk Buy now $99.00
QuEChERS Mixes
Sample Property QuEChERS Extraction Mixes QuEChERS clean-up mixes
Low fat content
(e.g., Apples, Strawberries)
Citrate or acetate extraction
Mix I or Mix II
Diamino clean-up
Mix III
Moderate content of chlorophyll and carotinoids
(e.g., Carrots, Lettuce)
Citrate or acetate extraction
Mix I or Mix II
Diamino/Carbon clean-up
Mix IV
Higher content of chlorophyll and carotinoids
(e.g., Bell Peppers, Spinach)
Citrate extraction
Mix I
Diamino/Carbon clean-up (higher Carbon content)
Mix V
Higher fat content
(e.g., Avocado)
Citrate extraction
Mix I
Diamino/C18 ec clean-up
CHROMABOND® QuEChERS Extraction Buffer Mixes
Catalog No. Description Composition Volume Qty/pkg
730970 Mix I Citrate Extraction Mix 4g MgSO₄, 1g NaCl, 0.5g Na₂H Citrate 1.5 H₂O, 1g Na₃ Citrate 2 H₂O 15 ml 50
730971 Mix II Acetate Extraction Mix 6g MgSO₄, 1.5g Na Citrate 15 ml 50
CHROMABOND® QuEChERS Clean Up Mixes
Catalog No. Description Composition Volume Qty/pkg
730972 Mix III Diamino Clean Up Mix 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ 15 ml 50
730973 Mix IV Diamino/Carbon Clean Up Mix 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ and 15 mg Carbon 15 ml 50
730974 Mix IV Diamino/Carbon Clean Up Mix 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ and 45 mg Carbon 15 ml 50
730975 Mix VI Diamino/C18 ec Clean Up Mix 0.15g CHROMABOND® Diamino with 0.9g MgSO₄ and 150 mg C18 ec 15 ml 50
CHROMABOND® Diamino Polypropylene SPE Cartridges
Catalog No. Description Weight Size Qty/pkg
730561 Diamino Adsorbent 200 mg 3 ml 50
730562 Diamino Adsorbent 500 mg 6 ml 30
CHROMABOND® Diamino Adsorbents
Catalog No. Description Qty/pkg
730653.20 Diamino Adsorbent 20g
730653 Diamino Adsorbent 100g