Reversed Phase TLC Plates

Gain the Advantage of Increased Selectivity, Speed of Development Time, Sharper Bands, and Greater Luminosity.

Our reversed phase TLC portfolio features three different C18 TLC plates, C2, Diol, Amino, and Cyano phases where functionalization of silica is modified using various short-chain organosilanes.

Retention of molecules and the ability to tolerate water are directly dependent on the organosilane chain length: the shorter the chain, the more water accepting and the shorter the migration time.

Our Diol and Cyano phases are moderately polar and can be used for both normal and reversed-phase modes. Our Amino phase has some ion-exchange character and is recommended for polar compounds.

Sorbtech Reverse Phase TLC Selection Guide
TLC Plate Type Description Features Types Available
C18-W HPTLC Plates
 Normal Phase or Reversed-Phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water.
  • Separation of very non-polar and highly polar substances using aqueous solvent systems.
  • Use to determine solvent system composition for downstream HPLC analysis. Provides additional selectivity’s as compared to Silica Gel plates.
  • Very fast migration times.
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- Plastic
✓ Aluminum
✓ Indicator
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C18-100 HPTLC Plates
 Octadecyl-Modified Nano Silica Layers, Complete (100%) octadecyl modification, carbon content 14%.
  • Reversed-phase separation mode with eluents from anhydrous solvents to mixtures with up to 20% water use for alkaloids, amino acids, barbiturates, polycyclic aromatic hydrocarbons (PAH), drugs, peptides, flavonoids, phenols, indole derivatives, steroids.
 ✓ Glass
- Plastic
- Aluminum
✓ Indicator
✓ w/o Indicator
C18-50 HPTLC Plates
 Octadecyl-Modified Nano Silica Layers; Partial (50%) octadecyl modification, carbon content 7.5%
  • Reversed-phase separation mode with eluents from anhydrous solvents to mixtures up to 60% of water Use for alkaloids, amino acids, barbiturates, polycyclic aromatic hydrocarbons (PAH), drugs, peptides, flavonoids, phenols, indole derivatives, steroids.
  • The 50% carbon load is less retentive than the C18-100.
 ✓ Glass
- Plastic
- Aluminum
✓ Indicator
✓ w/o Indicator
C2 TLC Plates
 Dimethyl-modified Nano Silica Layer. the C2 TLC plate can be developed with purely organic, organic/aqueous, and purely aqueous eluents.
  • Lower retention than C-18W coated plates. Moderately polar can be used in both normal and reversed-phase modes.
  • Exhibits higher polarity and high affinity of aqueous solutions, tolerating up to 80% water.
 ✓ Glass
- Plastic
✓ Aluminum
✓ Indicator
- w/o Indicator
Amino HPTLC Plates
 Aminopropyl modification, carbon content 3.5%
  • Moderately polar can be used in both normal and reversed-phase modes.
  • Use to separate hydrophilic or charged samples, Vitamins, sugars, steroids, purine derivatives, xanthine, phenols, nucleotides, and pesticides.
 ✓ Glass
- Plastic
✓ Aluminum
✓ Indicator
- w/o Indicator
Cyano HPTLC Plates
 Cyanopropyl modification, carbon content 5.5%
  • Moderately polar can be used in both normal and reversed-phase modes.
  • Use to separate hydrophilic or charged samples. Steroid hormones, phenols, preservatives.
 ✓ Glass
- Plastic
✓ Aluminum
✓ Indicator
- w/o Indicator
Diol HPTLC Plates
 Diol modification, carbon content 5.5%
  • Moderately polar can be used in both normal and reversed-phase modes.
  • Steroids, pesticides, and plant constituents, less sensitive than silica to the water environment.
 ✓ Glass
- Plastic
- Aluminum
✓ Indicator
- w/o Indicator