NUCLEOSHELL^® – PFP Pentafluorophenyl

• Pentafluorophenyl propyl modification
• Multi-end-capped
• Pore size 90 Å, particle size 2.7 µm, carbon
  content ~ 3%
• pH stability 1-9; suitable for LC/MS

• Aromatic and unsaturated compounds
• Phenols and halogenated compounds
• Isomers and polar compounds such as pharmaceuticals, antibiotics
• High retention of basic compounds

Fluorinated stationary phases in HPLC have gained increasing interest over the last years. The most common representative of fluorinated silica phases is the pentafluorophenyl modification (PFP or F5).

Especially the orthogonal selectivity compared to traditional alkyl phases widens the scope in analytical HPLC. NUCLEOSHELL PFP offers excellent selectivity especially for:

• Highly polar analytes, aromatic, and unsaturated compounds
• Phenols or halogenated hydrocarbons

Halogen substitutes in molecules result often in an increase of their polarity accompanied by a decrease of typical retention characteristics in RPHPLC.

While a typical C18 phase just provides hydrophobic interactions between the stationary phase and analyte, NUCLEOSHELL^® PFP offers four different retention mechanisms:

• Polar interactions (H bonds)
• Dipoledipole interactions
• π-π interactions
• Hydrophobic interactions

Especially the pronounced ion exchange capacity and distinct steric selectivity are typical for the character of fluorinated phases.

NUCLEOSHELL^® PFP combines the benefits of core-shell technology, high stability, and orthogonal selectivity. This makes it a useful complementary tool for highly efficient separations especially of isomers, halogenated, aromatic and/or polar compounds.