NUCLEODEX^®

Nucleodex comprises of cyclodextrin-based phases such as β-OH, α-PM, β-PM, and γ-PM.

These chiral cyclodextrin phases are suited for the separation of racemates, constitutional and configurational isomers under reversed-phase conditions.

Numerous racemates, including pesticides and pharmaceuticals, are separated using these phases which are based on spherical silica Nucleosil^®.

Nucleodex columns enantiomer separation based on cyclodextrins

• Base material Nucleosil® silica, particle size 5 µm, pore size 100 Å
• Modified cyclodextrins as chiral selectors

Nucleodex β-OH: β-cyclodextrin (R = H; n = 2)-USP L45

• Separation based on hydrogen bonds and dipole interactions between functional groups of the analyte and hydroxyl groups of the cyclodextrin
• Successful enantiomer separations: chlorthalidone and other compounds, which require free hydroxyl groups for enantioselective interactions
• Eluent in column CH₃OH / 0.1 % TEAA pH 4 (55:45)

Nucleodex α-PM: permethylated α-cyclodextrin (R = CH₃; n = 1)

• For all permethylated phases, the ability to form hydrogen bonds is reduced, the hydrophobicity of the phase is increased compared to β-OH, resulting in shorter retention times
• Examples for successful enantiomer separations: mecoprop and dichlorprop as free carboxylic acids, trans-stilbene oxide, styrene oxide
• Eluent in column CH₃OH / 50 mM phosphate pH 3 (70:30)

Nucleodex β-PM: permethylated β-cyclodextrin (R = CH₃; n = 2)-USP L45

• Examples for successful enantiomer separations: mephobarbital (prominal) pesticide derivatives mecoprop methyl and dichlorprop methyl
• Eluent in column CH₃OH / 0.1 % TEAA pH 4 (65:35)

Nucleodex γ-PM: permethylated γ-cyclodextrin (R = CH₃; n = 3)

• Successful enantiomer separations: steroids or other larger molecules
• Eluent in column CH₃OH / 0.1% TEAA pH 4 (55:45)

Nucleodex phases are especially suited for the control of optical purity, but also for semi-preparative separations and for the analysis of positional and cis-trans isomers.