Bioseparations – Wide Pore Hydrophilic Anion

Ion Exchange Resins, Sepabeads Resin Weak Base Methacrylic Anion Exchanger 600A 75-150µm 1.0 meq/ml Chloride Form Water Content: 41-47%

The CQ grade resins are based on a highly crosslinked polymethacrylic matrix, which is further hydroxylated for added hydrophilicity.

These matrices are functionalized with either a quaternary amine (trimethyl amine) or a diethyl-aminoethyl to form strong and weak basic anion exchangers with a chloride counterion.

By varying the bead size of these resins, you can control the efficiency and resolution when separating anionic proteins, peptides, and polynucleotides (separation ran at a pH > PI of the target molecule).

These Methacrylic Matrices Have:

  • Excellent mechanical strength
  • Wide range pH stability (1 to 13)
  • Resistance to high temperatures (up to 60°C operating temperature)

The FP Grade Resins Are:

  • Based on highly crosslinked polymethacrylic matrix, which is hydroxylated for added hydrophilicity
  • Functionalized with diethyl-amino, or hexyldiamine to form weak basic anion exchangers
  • Used in the large scale separation of anionic proteins, peptides, and polynucleotides (separation ran at a pH > PI of the target molecule)
  • Suited for enzyme immobilization supports

These methacrylic matrices have excellent mechanical strength with wide range pH stability (1 to 13) and resistance to high temperatures (up to 60° C operating temperature).

Diethylamine (DEA) functionality is a secondary amine base group which provides increased capacity towards
target molecules.