Features:

  • Based on core-shell particle technologyfor fast and efficient HPLC
  • Suitable for LC/MS and HPLC at pH extremes (pH 1-11)
  • Superior base deactivation, ideal for method development

Technical Characteristics:
Octadecyl modification, multiendcapped; pore size 90 Å, particle size 2.7 µm, carbon content 7.5%

Recommended application:
Overall sophisticated analytical separations, e.g., analgesics, anti inflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosuppressants

NUCLEOSHELL RP 18 combines innovative silica technology and excellent surface deactivation, that outperforms conventional C18 silicas in terms of efficiency, resolution and speed. Due to the applied core-shell particle design the back pressure at elevated flow rates remains at a moderate level and permits the use of existing HPLC equipment in many cases. NUCLEOSHELL RP 18 with extended pH stability, low bleed characteristics in LC/MS applications and overall robustness is an ideal tool for method development and routine analysis in modern HPLC.

NUCLEOSHELL RP 18 is based on core-shell particle technology silica. A unique derivatization process generates a homogeneous surface with a high density of bonded silanes (carbon content ~7.5%). Endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes NUCLEOSHELL RP 18 particularly suitable for the separation of basic and other ionizable analytes. The extremely reduced silanol activity of the phase can be demonstrated by applying basic analytes, such as tricyclic antidepressants.

Tanaka plot of NUCLEOSHELL RP 18 The diagram below underlines the distinct hydrophobic characteristics and the low silanol activity of the stationary phase.


Capacity = k'(pentylbenzene)
Hydrophobicity = α(pentylbenzene, butylbenzene)
Steric selectivity = α(triphenylene, o-terphenyl)
Hydrogen bonding capacity (silanol capacity) = α(caffeine, phenol)
Ion exchange capacity at 2 different pH values (2.7 and 7.6) = α(benzylamine, phenol)









Catalog #/DescriptionAdd to Cart/List Price
763132.20 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 2x50mmAdd to Cart  649.00
763132.30 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 3x50mmAdd to Cart  650.00
763132.40 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4x50mmAdd to Cart  650.00
763132.46 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4.6x50mmAdd to Cart  708.00
763134.20 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 2x100mmAdd to Cart  742.00
763134.30 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 3x100mmAdd to Cart  754.00
763134.40 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4x100mmAdd to Cart  754.00
763134.46 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4.6x100mmAdd to Cart  822.00
763136.20 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 2x150mmAdd to Cart  742.00
763136.30 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 3x150mmAdd to Cart  754.00
763136.40 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4x150mmAdd to Cart  754.00
763136.46 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4.6x150mmAdd to Cart  822.00


Guard Cartridges

Catalog #/DescriptionAdd to Cart/List Price
763138.20 - MN HPLC Guard Cartridges, Nucleoshell RP 18, 90A, 2.7um, 2x4mm, 3/pkAdd to Cart  143.00
763138.30 - MN HPLC Guard Cartridges, Nucleoshell RP 18, 90A, 2.7um, 3x4mm, 3/pkAdd to Cart  143.00
Application #Application TitleLink
124880Stability of NUCLEOSHELL RP 18 under acidic and basic conditions
124910Batch to batch reproducibility of NUCLEOSHELL RP 18
124920Accelerating the separation of aromatic ketones by use of core-shell particles
124921Separation of aromatic ketones
124930Comparison of the separation of steroids on NUCLEOSHELL RP 18 and on NUCLEODUR® C18 Gravity
124931Separation of steroids
124940Comparison of the separation of ß-lactam antibiotics on NUCLEOSHELL RP 18 and on NUCLEODUR® C18 Gravity
124941Separation of ß-lactam antibiotics
124950Separation of tricyclic antidepressants on NUCLEOSHELL RP 18 compared with NUCLEODUR® C18 Gravity, 5µm
124951Separation of tricyclic antidepressants
124960Separation of antidepressants on NUCLEOSHELL RP 18 compared with competitor phases
124970Separation of nonsteroidal anti-inflammatory drugs on NUCLEOSHELL RP 18 compared with a competitor phase with similar modification
124971Separation of nonsteroidal anti-inflammatory drugs
125270Resolution as a function of particle size: Separation of naphthalene and ethyl benzene
125400Temperature stability of NUCLEOSHELL RP 18, 2.7 µm
125410Batch-to-batch reproducibility
125420Saving time and solvent by using fused-core particles
125430Accelerating the separation of acidic pharmaceuticals by the use of core-shell particles
125500Comparing the efficiency of NUCLEOSHELL RP 18 with different fused-core phases and fully porous phases
125510Comparing the pressure drop of core-shell phases
125520Stability of NUCLEOSHELL RP 18 under acidic conditions compared with competitor phase
125530Stability of NUCLEOSHELL RP 18 under basic conditions compared with competitor phases
125540Comparing the peak capacity of NUCLEOSHELL RP 18 with NUCLEODUR® C18 Gravity
125621Separation of steviol glycosides on NUCLEOSHELL RP 18, 2.7 µm
125720Separation of steviol glycosides on NUCLEOSHELL RP 18, 2.7 µm with MS detection
125730Separation of steviol glycosides on NUCLEOSHELL RP 18, 2.7 µm with ELSD detection
125770Separation of carbonyl dinitrophenylhydrazones
126110Comparison of liquid chromatography columns for the quantification of glyphosate, glufosinate and AMPA using LC-UV-MSMS – NUCLEOSHELL® RP 18
126240Separation of tocopherols with NUCLEOSHELL® RP 18
126630Separation of cephalosporin antibiotics - Comparison of 5 µm core-shell and totally porous phase
126640Bleeding characterisitics of NUCLEOSHELL® RP 18plus compared with competitor phases
126650pH stability of NUCLEOSHELL® RP 18plus
126660Polar selectivity shown for vancomycin
126670Phase collapse and regeneration
126680Separation of pharmaceuticals compared with a competitor phase
126690Comparison of steric selectivity
126700Comparing the separations of organic acids
126710Comparing the separations of sweeteners
126720Comparing the separations of ß-lactam antibiotics
126730Comparing the separations of water-soluble vitamins
126830Endcapping stability of NUCLEOSHELL® RP 18plus
126840Comparing NUCLEOSHELL® RP 18plus and NUCLEOSHELL® RP 18 in the separation of acidic pharmaceuticals
126850Comparing NUCLEOSHELL® RP 18plus and NUCLEOSHELL® RP 18 in the separation of tricyclic antidepressiva
126860Medroxyprogesteron acetate according to Ph. Eur.
126870Amlodipine besilate according to Ph. Eur.
126880Azithromycin according to Ph. Eur.
126910Bifonazole according to Ph. Eur.
126920Comparing NUCLEOSHELL® RP 18plus and NUCLEOSHELL® RP 18 in the separation of organic acids
126930Cefepime dihydrochloride according to Pharm. Eur.
126940Comparing NUCLEOSHELL® RP 18plus and NUCLEOSHELL® RP 18 in the separation of steroids
126950Determination of betamethasone dipropionate according to Pharm. Eur.
126960Determination of bisacodyl according to Pharm. Eur.
126970Determination of atropine according to Pharm. Eur.
126980Determination of synthetic antioxidants according to AMG
126990Determination of atenolol according to Pharm. Eur.