NUCLEOSHELL^® – HILIC

• Based on core-shell particle technology for fast and efficient HPLC
• Ideal for reproducible and stable chromatography of highly polar analytes
• Very short column equilibration times

• Ammonium – sulfonic acid-modified silica
• Pore size 90 Å, particle size 2.7 µm
• Carbon content 1.3%
• pH stability 2-8.5
• Suitable for LC/MS

Hydrophilic compounds such as organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water-soluble vitamins

This a separation technique using polar stationary phases and organic-aqueous mobile phases. The minimum water content of at least 2% is indispensable to provide a permanent water layer between the adsorbent surface and the organic fraction of the mobile phase.

The sample molecules become separated in a partition chromatography, in which polar analytes are more strongly retained than neutral, less hydrophilic compounds.

Consequently, increasing the aqueous part in the mobile phase will diminish the retention of the polar sample constituents. In this way, HILIC behaves inverse to classical RP chromatography.

The retention profile of HILIC enables the chromatography of very polar and often small molecules, which won’t show any retention on C8 or C18 reversed phases.