Features:

  • Ideal for reproducible and stable chromatography of highly polar analytes
  • Suitable for analytical and preparative applications as well as LC-MS
  • Very short column conditioning period

Technical Characteristics:
Ammonium-sulfonic acid modified silica; pore size 110 Å; particle sizes 1, 8, 3, and 5 µm; carbon content 7%; pH stability 2-8.5

Recommended Application:
Hydrophilic compounds such as organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins

Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC-the most common analytical method-is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC.

HILIC combines the characteristics of the 3 major methods in liquid chromatography-reversed phase (RPC), normal phase (NPC) and ion chromatography (IC):

  • Stationary phases (adsorbents) are mostly polar modifications of silica or polymers (SiOH, NH2, Diol, (zwitter) ions,…)-like in NPC
  • Mobile phases (eluents) are mixtures of aqueousbuffer systems and organic modifier like acetonitrile or methanol-like in RPC
  • Fields of application include quite polar compounds as well as organic and inorganic ions-like in IC

NUCLEODUR® HILIC is a special zwitterionic modified stationary phase based on ultra spherical NUCLEODUR® particles. The betaine character of the ammonium-sulfonic acid ligands results in total charge equalisation and in an overall neutrally charged but highly polar surface.

Retention characteristic
Commonly HILIC is described as partition chromatography or liquid/liquid extraction system between the mobile and stationary phase. Versus a water-poor mobile phase a water-rich layer on the surface of the polar stationary phase is formed. Thus, a distribution of the analytes between these two layers will occur. Furthermore HILIC includes weak electrostatic mechanisms as well as hydrogen donor interactions between neutral polar molecules under high organic elution conditions. This distinguishes HILIC from ion exchange chromatography-main principle for HILIC separation is based on compound's polarity and degree of solvation. More polar compounds will have stronger interaction with the stationary aqueous layer than less polar compounds-resulting in a stronger retention.




Nonpolar compounds exhibit faster elution profiles due to minor hydrophobic interactions. Thus, as shown for the separation of uracil and naphthalene the elution order is quite often inverse on HILIC columns compared to RP columns.

In comparison with medium polar aminopropyl phases or modification with less balanced charge equalisation NUCLEODUR® HILIC shows a superb separation and peak shape for critical compounds like adenosine phosphates.

Stability Features
Due to an advanced and unique surface modifi cation procedure (pat. pend.) NUCLEODUR® HILIC columns provide short equilibration times-after just 20 min equilibration already the 2nd injection shows stable and reproducible results. Beyond this, NUCLEODUR® HILIC columns are characterized by an outstanding column life time-even after nearly 800 runs the columns show no loss of pristine performance-peak shape and retention are still immaculate.

Due to its high loadability NUCLEODUR® HILIC is absolutely suitable for preparative and semi-preparative applications.



Catalog #/DescriptionAdd to Cart/List Price
760521.20 - MN HPLC Column, Nucleodur HILIC, 110A, 1.8um, 2x30mmAdd to Cart  662.00
760521.30 - MN HPLC Column, Nucleodur, Hilic, 110A, 1.8um, 3x30mmAdd to Cart  663.00
760521.40 - MN HPLC Column, Nucleodur HILIC, 110A, 1.8um, 4x30mmAdd to Cart  663.00
760521.46 - MN HPLC Column, Nucleodur HILIC, 110A, 1.8um, 4.6x30mmAdd to Cart  723.00


HILIC Guard Columns

Catalog #/DescriptionAdd to Cart/List Price
761960.20 - MN HPLC Guard Cartridges, Nucleodur Hilic, 1.8um, 2x4mm, 3/pkAdd to Cart  138.00
761960.30 - MN HPLC Guard Cartridges, Nucleodur Hilic, 1.8um, 3x4mm, 3/pkAdd to Cart  138.00
761961.20 - MN HPLC Guard Cartridges, Nucleodur Hilic, 3um, 2x4mm, 3/pkAdd to Cart  138.00
761961.30 - MN HPLC Guard Cartridges, Nucleodur Hilic, 3um, 3x4mm, 3/pkAdd to Cart  138.00
761962.20 - MN HPLC Guard Cartridges, Nucleodur Hilic, 5um, 2x4mm, 3/pkAdd to Cart  138.00
761962.30 - MN HPLC Guard Cartridges, Nucleodur Hilic, 5um, 3x4mm, 3/pkAdd to Cart  138.00
Application #Application TitleLink
122912Comparison of NUCLEODUR® HILIC with C18 Phase
122920Separation of nucleotides on NUCLEODUR® HILIC
122930Separation of organic acids on NUCLEODUR® HILIC
122940Separation of ascorbic acid and dehydroascorbic acid on NUCLEODUR® HILIC
122950Separation of Pyrimidine and Purine Bases on NUCLEODUR® HILIC
122960Separation of Vitamin B7 and B12 on NUCLEODUR® HILIC
122970Separation of water soluble vitamins on NUCLEODUR® HILIC
122980Separation of Aromatic Amino Acids and Histamine on NUCLEODUR® HILIC
122990Separation of Creatinine and Creatine on NUCLEODUR® HILIC
123000Separation of Creatinine and Creatine on NUCLEODUR® HILIC with LC-MS Detection
123010Separation of Acrylamide, Methacrylamide and Methacrylic Acid on NUCLEODUR® HILIC
123020Separation of the Chemotherapeutic Agent 5-Fluorouracil and Uracil on NUCLEODUR® HILIC
123030Separation of Catecholamines on NUCLEODUR® HILIC
123040Separation of Chlormequat and Mepiquat on NUCLEODUR® HILIC with LC-MS Detection
123050Separation of Paraquat and Diquat on NUCLEODUR® HILIC
123060Separation of Paraquat and Diquat on NUCLEODUR® HILIC with LC-MS Detection
123070Separation of melamine and cyanuric acid on NUCLEODUR® HILIC with UV and LC-MS detection
123090Determination of melamine in milk on NUCLEODUR® HILIC with UV and LC-MS detection
123100Relative Lifetime of NUCLEODUR® HILIC
123170Separation of Ethyl Glucuronide and Ethyl Sulphate on NUCLEODUR® HILIC
123350Determination of Glyphosate, Glufosinate and AMPA with HPLC-MS
123490Comparison of NUCLEODUR® HILIC with SeQuantTM ZIC®-HILIC for Acrylamide Separations
123500Comparison of NUCLEODUR® HILIC and SeQuantTM ZIC®-HILIC for Separations of Purine and Pyrimidine Bases
123510Comparison of NUCLEODUR® HILIC with SeQuantTM ZIC®-HILIC and Phenomenex LUNA® HILIC for Hydroxybenzoic Acids Separations
123940Separation of Guanosine Phosphates
123960Separation of Guanosine and Inosine Monophosphates
124030Comparison of 3 µm with 1.8 µm NUCLEODUR® HILIC phase for naphthalene and nucleic acid bases
124040Ultra-fast HPLC separation of aromatic amino acids on NUCLEODUR® HILIC, 1.8 µm
124050Comparison of 3 µm with 1.8 µm NUCLEODUR® HILIC phase for creatinine and creatine
124060Ultra-fast HPLC separation of aromatic acids on NUCLEODUR® HILIC, 1.8 µm
124070Ultra-fast HPLC separation of metformin and dicyandiamide on NUCLEODUR® HILIC, 1.8 µm
124080Ultra-fast HPLC separation of vitamin B6, levocarnitine and gamma-butyrobetaine on NUCLEODUR® HILIC, 1.8 µm
124320Separation of Urea and Hydroxyurea
124350Determination of Allantoin by HPLC
125330Quantitative determination of ethyl glucuronide and ethyl sulfate from urine by LC-MS/MS
125740Separation of steviol glycosides by hydrophilic liquid interaction chromatograpy