Clarion S25M Series
Hydrated gel filtration columns for protein purification and desalting.

• Clarion 2-S25M processes sample volumes of 150 to 300 µL
• Clarion 5-S25M processes sample volumes of 0.5 mL
• Clarion 10-S25M processes sample volumes of 0.5 mL to 1.0 mL
• Clarion 25-S25M processes sample volumes of 2.5 mL
• Clarion 50-S25M processes sample volumes of 5 mL
• Clarion 100-S25M processes sample volumes of 10 mL

Clarion Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins.

The gel matrix of Clarion S25M is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Sorbadex-25 is 5 kD for proteins.

• Clarion 2-S25M – Proteins larger than 5 kD in a sample volume of up to 200 µL can be purified with an elution volume of 200 to 350 µL.
• Clarion 5-S25M – Proteins larger than 5 kD in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.
• Clarion 10-S25M – Proteins larger than 5 kD in a sample volume of up to 1 mL can be purified with an elution volume of 1.2 to 1.5 mL.
• Clarion 25-S25M – Proteins larger than 5 kD in a sample volume of up to 2.5 mL can be purified with an elution volume of 2.7 to 3.5 mL.
• Clarion 50-S25M – Proteins larger than 5 kD in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.
• Clarion 100-S25M – Proteins larger than 5 kD in a sample volume of 10 mL can be purified with an elution volume of 12 to 15 mL.

Clarion S50M Series
Hydrated gel filtration columns for protein purification and desalting.

• Clarion 2-S50M processes sample volumes of 150 to 300 µL
• Clarion 5-S50M processes sample volumes of 0.5 mL
• Clarion 10-S50M processes sample volumes of 0.5 mL to 1.0 mL
• Clarion 25-S50M processes sample volumes of 2.5 mL
• Clarion 50-S50M processes sample volumes of 5 mL
• Clarion 100-S50M processes sample volumes of 10 mL
• Clarion 500-S50M processes sample volumes of 50 mL

The gel matrix of Clarion is Sorbadex-50, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.

Molecules purified with Sorbadex-50 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.

The gel matrix of Clarion is Sorbadex-50, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Sorbadex-50 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Sorbadex-50 is 20 base pairs for oligonucleotides.

• Clarion 2-S50M – Nucleic acids larger than 20 bp in a sample volume of up to 200 µL can be purified with an elution volume of 200 to 350 µL.
• Clarion 5-S50M – Nucleic acids larger than 20 bp in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.
• Clarion 10-S50M – Nucleic acids larger than 20 bp in a sample volume of up to 1 mL can be purified with an elution volume of 1.2 to 1.5 mL.
• Clarion 25-S50M – Nucleic acids larger than 20 bp in a sample volume of up to 2.5 mL can be purified with an elution volume of 2.7 to 4.0 mL.
• Clarion 50-S50M – Nucleic acids larger than 10 bp in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.
• Clarion 100-S50M – Nucleic acids larger than 10 bp in a sample volume of 10 mL can be purified with an elution volume of 12 to 15 mL.
• Clarion 500-S50M – Nucleic acids larger than 10 bp in a sample volume of 50 mL can be purified with an elution volume of 65 to 70 mL.