Polyamide 6 – High adsorption for Prep Chromatography

Polyamide 6 is widely used to separate botanical extracts and other natural products that contain phenolic and polyphenolic groups.

Because of its swelling properties, Polyamide 6 provides a very high adsorption capacity. In fact, it performs much better than silica gel, alumina, PSDVB, and most other sorbents available today.

Moreover, it is ideally suited for preparative chromatography, where large-scale separations are needed. At the same time, it is also valuable for analyzing phenolic and polyphenolic compounds in natural product research. As a result, Polyamide 6 offers both versatility and strong performance across many chromatography applications.

Polyamide 6 Chromatography Applications:

It is commonly used to isolate and identify natural compounds that contain phenolic and polyphenolic functional groups, such as:

  • Flavonoids
  • Anthocyanins
  • Anthoxanthines
  • Anthraquinones
  • Flavones and flavonols
  • Isoflavones

In summary, this makes it especially valuable for natural product research and analysis.

Polyamide 6 – High adsorption for Prep Chromatography

By adjusting the balance between adsorption and partition effects, you can change the retention behavior of polyamide in many ways. Because polyamide has medium polarity, the stationary phase (polyamide swollen with eluent) works well with mobile phases that are either more polar or less polar. As a result, this flexibility makes it possible to run two-dimensional chromatography using the same stationary phase, combining both normal-phase and reversed-phase techniques.

Adsorption Depends On:

  1. The number of the polar groups,
  2. The degree of the H-bonding activities,
  3. Size of the analytes, and
  4. The shape of the analytes.

Thus, structural isomers can be well separated on polyamide.

Typical Polyamide 6 Applications:

  • Phenols, aromatic nitro, and amino compounds
  • Chalcones, chinones, flavones, and anthraquinones
  • Anthocyanins and anthoxanthines
  • DNP-amino acids
  • Carbonic acids and their Amides
  • Sulfonic acids and their amides