This post highlights key chromatographic metrics—retention factor (k), selectivity (α), efficiency (N)—that collectively determine separation quality, as measured by resolution (Rₛ) Sorbent Technologies, Inc.+1.
In practical tests, three silica grades—Technical (40930), Standard (40930H), and Enhanced (40930M)—were compared using acetophenone mixtures on a 40g column at 30 mL/min Sorbent Technologies, Inc.+1.
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Enhanced (40930M) delivered the best selectivity and resolution, though its efficiency was slightly lower due to lower water content.
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Standard (40930H) offered better efficiency than Technical, striking a good balance of performance.
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Technical (40930) performed poorest, with broader specifications compromising separation Sorbent Technologies, Inc.+1.
When testing higher sample loads (up to 5%), both Standard and Enhanced outperformed Technical. Baseline resolution became achievable with 40930H and 40930M, but not with 40930 Sorbent Technologies, Inc.+1.
Beyond those, two premium-grade silicas, 52500 and 52300, were evaluated. Both feature high purity and tight particle distribution, resulting in superior selectivity, resolution, and efficiency—outperforming the 40930 series Sorbent Technologies, Inc.+1.
Between the two, the smaller particles of 52300 provided dramatically sharper peaks and better efficiency, making it ideal when even with 5% loading, clear baseline separation is desired Sorbent Technologies, Inc.+1.
Key Takeaways:
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40930H is recommended over the basic grade for better overall performance.
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40930M excels when selectivity and low water content are critical.
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52500 and 52300 set a higher standard and are best for demanding separations, especially 52300 when tight efficiency is key.